The Animal House facility maintains and provides animal stocks for faculty in both the biology and psychology programs and remains under the direction of the Biology Department. Its daily operation is carried out by half-time animal health technician and laboratory animal technologist Cindy Chrisler.
The Electron Microscope and Image Analysis Center (EMIAC) is located in the School of Natural Sciences at California State University at San Bernardino. The Center includes a Scanning Electron Microscope (SEM), a Transmission Electron Microscope (TEM) and sample preparation facilities. In the Fall of 1992, the Department purchased a Hitachi S-2700 Scanning Electron Microscope (SEM) with a backscatter detector, on-line image measurement system, a thermal video printer and a water chiller. The SEM allows ultrastructural resolution of the surface of samples at magnifications of 35X to 50,000X. The image obtained is a three-dimensional image of the surface structure of the specimen. Specimen preparation equipment also acquired at that time included a Hummer VI-A sputter coater with carbon coating accessory and a Tousimis Samdri-780A critical point dryer.
Funding for this phase was provided by an Instrumentation and Laboratory Improvement (ILI) award of $62,645 from the National Science Foundation to Drs. Thompson, Brasch and Fehn in Biology and Dr. Leatham in Geology. This was matched with funds from the University.
In the Fall of 1993, a Zeiss EM-900 transmission electron microscope (TEM) was purchased with a film dessicator. Funding for this phase came from the University. For internal morphology, the TEM is capable of magnifications of 1,000X to 100,000X.
The School renovated a suite of rooms located in the basement of the Biology Building to house the EMIAC. The facility includes an SEM room, a TEM room, a specimen preparation room, a darkroom and a light microscope room. The light microscope room houses a fluorescence microscope, a polarizing microscope and a phase-contrast microscope.
Image capture is available through thermal video printer on the SEM, Polaroid prints and/or negatives on the SEM, photo negatives on the TEM, and videotape recording of the SEM. The videotape recording system includes two Panasonic Super-VCR's to allow for editing and a S-video Panasonic monitor. We are also able to project images from the light or scanning electron microscopes to classrooms throughout the University.
A course for students (BIOL 530 or GEOL 530) teaches hands-on use of the SEM and has been taught for several years. Students and faculty are currently utilizing the SEM for research projects. A course which will train students to use the TEM is being planned. Future developments of the Center include the acquisition of digital capture of images from both the SEM and TEM. It will then be possible to analyze the digitized images with image analysis software. The digitized images will also be available for transmission to remote sites via Internet connections.