Assistant Professor of Biology
B.S. Zoology, National Taiwan University
Ph.D. Genetics and Development, University of Texas Southwestern Medical Center
Postdoctoral fellow in Neuroscience, Massachusetts General Hospital and Harvard Medical School
BIOL 300 - Cell Physiology
My research interests are in understanding the molecular basis of animal behavior, particularly in understanding neural plasticity at the molecular and cellular levels. If someone pokes you with their finger, you respond differently if they poke you from the front, or if they surprise you from the back. How are neural circuits controlled so that we respond differently to a given stimulus? What are the genes that are involved in this process?
In my lab we use the nematode Caenorhabditis elegans to study the molecular and cellular basis of chemosensory behavior and how it is regulated by environmental conditions. C. elegans is a powerful model system that is used to study molecular and behavioral neuroscience, because of its extremely simple and well-described nervous system. The adult C. elegans (~1 mm long) has only 302 neurons in its entire nervous system. Furthermore, the "wiring" of its entire nervous system is known down to the level of individual synapses. Finally, C. elegans is highly amenable to experimental manipulation. In my lab, we use various molecular, cellular, behavioral, and genetic techniques, including generating transgenic animals, laser microsurgery, and recombinant DNA.
We are currently interested in understanding how the neurotransmitters serotonin and dopamine contribute to the regulation of C. elegans chemosensory behavior. Serotonin and dopamine are critical in regulating human behaviors and behavioral disorders, such as bulimia and anorexia, bipolar disorder, schizophrenia, drug abuse, and others. By studying a simple model organism such as C. elegans, we hope to gain insight into understanding how the nervous systems of more complex animals function. Several C. elegans genes, including gpa-11, dgk-1, and glr-1, appear to be important for controlling C. elegans behavior. We are taking genetic and behavioral approaches to further understanding the roles of the genes.
1. Komatsu, H., Chao, M.Y., Larkins-Ford, J., Dionne, H.M., Tucey, T.M., Mishra-Gorur, K., White, J., Artavanis-Tsakonas, S., and Hart, A.C. C. elegans SEL-14 encodes a Notch ligand required for development. In review.
2. Chao, M.Y., Larkins-Ford, J., Tucey, T.M., and Hart, A.C. lin-12 Notch functions in the adult nervous system. BMC Neuroscience (2005) 6:45.
3. Chao, M, Y., Komatsu, H., Fukuto, H.S., Dionne, H.M., and Hart, A.C. Feeding status and serotonin rapidly and reversibly modulate a C. elegans chemosensory circuit. Proc. Natl. Acad. Sci. USA 101, 15512-15517 (2004).
4. Chao, M.Y., and Hart, A.C. Sensory biology: how the nose knows. Curr. Biol. 13, R226-R228 (2003).
5. Huang, H.-R., Chao, M.Y., Armsrtong, B., Wang, Y., Lambowitz, A.M., and Perlman, P.S. A high affinity maturase binding site in a group II intron is essential for intron homing but not for in vivo splicing. Mol. Cell. Biol. 23, 8809-8819 (2003).
6. Eskes, R., Liu, L., Ma, H., Chao, M.Y., Dickson, L., Lambowitz, A.M., and Perlman, P.S. Multiple homing pathways used by yeast mitochondrial group II introns. Mol. Cell. Biol. 20, 8432-46 (2000).
7. Chao, M.Y., Kan, M.C., and Lin-Chao, S. RNAII transcribed by IPTG-induced T7 RNA polymerase is non-functional as a replication primer for ColE1-type plasmids in Escherichia coli. Nucl. Acids Res. 23, 1691-1695 (1995).