Lab Pics

Lab Field Trips



Ruth and Tomasz
RWR (2010) Ruth and Tomasz.
Johnny, Jessica, Krista and Nelson
RWR (2012) Johnny, Jessica, Krista and Nelson.


Mark, Jessica, Ruth, Dorothy, Nelson and Johnny
RWR (2013) Mark, Jessica, Ruth, Dorothy, Nelson and Johnny.
Nelson, Mark, Jessica, Dorothy and Johnny
CSUSB (2013) Nelson, Mark, Jessica, Dorothy and Johnny.


Jaime, David, Ruth and Alberto
RWR (2014) Jaime, David, Ruth and Alberto.
Gator with flowers
Silent and deadly, among pretty flowers.... © Ruth Elsey


Gator by nest
Gator protecting her "next egg" investment. © Ruth Elsey
Gator mama en garde
Don't come any closer! © Ruth Elsey


Nest raider
Dr O, Nest Raider.
David pinching eggs
All you need is a bucket and a stick.


Egg packing
Packing eggs at RWR.
Egg unpacking
Unpacking eggs at CSUSB.
Eggs in the gator-bator
Eggs in the Gator-bator at CSUSB.


EGG EXPERIMENTS



Egg with embryo
Egg with embryo in situ,
seen through the chorioallantoic membrane.
Eggs with and without eggshell
Alligator eggs with (left)
and without (right) their calcareous eggshell.
Gator embryos
Cleared and stained embryos incubated with (left)
and without (right) the eggshell.
Blue is cartilage, dark crimson - bone.
Note reduced mineralisation of the skeleton
in the experimental embryo.


Eggshell SEM
Scanning electron micrograph of the alligator eggshell.
EDS analysis of the eggshell
EDS analysis of the eggshell fragment elemental composition.


Gator with strong bite
Alligator hatchlings incubated in control eggs
have stiff bones
and produce strong bite forces.
Gator with weak bite
Alligator hatchlings incubated in experimental eggs
(without eggshells) have "bendy bones"
and produce weak bite forces.


BONE MICROSTRUCTURE



Gator on treadmill
Alligator gets a workout on a treadmill.
Gator growth
Alligator growth is tracked weekly by dedicated students.


Gator femur
Mid-diaphyseal femoral cross-section
of a juvenile female alligator,
with growth rings of calcein (yellow) and alizarin (red).
For a high resolution image, click here.
Gator femur image
Femoral cortex from a hatchling alligator
raised under chronic hyperoxia, with fluorescent dyes
incorporated in the matrix.
For a high resolution image, click here.


Gator distal femur trabeculae
Longitudinal section of the alligator femur
showing the primary and secondary spongiosa of the distal metaphysis.
For a high resolution image, click here.
Gator proximal femur
Longitudinal section of the alligator femur
under UV illumination, highlighting the growth and remodelling of the trabeculae.
For a high resolution image, click here.


HEAT EXCHANGE, RESPIRATORY TURBINATES AND BREATHING PATTERNS



Bandsaw histology
Alligator mississippiensis. Bandsaw histology of the adult alligator skull.
Emu
Dromaius novaehollandiae. Emus have the most complex
respiratory turbinates of all modern birds.
They're also big and hard to tackle....


Sagittal section of juvenile alligator head
Alligator mississippiensis. Sagittal section of a juvenile alligator head,
reveals a prominent preconcha in the nasal cavity.
Sagittal section of an adult ostrich head
Struthio camelus. Sagittal section of an adult ostrich head
showing the complex conchae.


Thermographic image of Varanus nose
Varanus exanthematicus.
Thermographic image (taken with a FLIR camera) of the nose
of a juvenile savannah monitor, reveals a cold eye and a warm nostril.
Iguana thorax
Iguana iguana.
Ribcage of lizards is highly compliant, with thin ribs,
long costal cartilages and a short sternum.


Armadillo skeleton
Euphractus sexcinctus. Robust ribs confer stiffness on the mammalian thorax.
Mystery sternum
Mystery sternum. Guess the species!


CARDIAC SHUNTS AND REPAIR



Outflow tract with shunt
Cross-section of the outflow tract
from an alligator heart,
showing three major arteries: right aorta (left),
pulmonary artery (right), and left aorta (middle).
Verhoeff stain.
Outflow tract without shunt
Cross-section of the outflow tract
from an alligator heart,
with the left aorta surgically occluded.
Note its small lumen.
Verhoeff stain.
Thermographic image with and without shunt
Thermographic image of two juvenile alligators,
one with and one without the pulmonary bypass shunt.
Can you tell which one is which?


Trabecular myocardium of an alligator heart
Trabecular myocardium of an alligator heart,
following LAo occlusion.
Note the proportion of muscle (red)
and connective tissue (blue).
Masson's trichrome stain.
Alligator heart
Longitudinal section of an alligator heart
following apical amputation of the left ventricular wall.
AFOG stain.
Cardiomyocyte proliferation
Cardiomyocyte proliferation in the injury border-zone
of an alligator left ventricle.
Mef2/ PHH3/BrdU stain.


LAB OUTSIDE OF LAB



SouthWest DVM 2013 at UC Riverside
SouthWest DVM 2013 at UC Riverside.
Experimental Biology 2014
Experimental Biology 2014 in San Diego:
Jessica, Nelson, Alyssa, David, Krista, Johnny, Tomasz.


Young scientist with gator
Lab volunteers of all ages always welcome!
Henry's artwork
Gators shall inherit the Earth! © Henry Tsai