Talbot, Colleen

Dr. Colleen Talbot

Associate Professor

e-mail: ctalbot@csusb.edu Image of frog

Biology Department
California State University
San Bernardino, CA 92407

B.S. California State Polytechnic University, Pomona
M.S. California State Polytechnic University, Pomona
Ph.D. University of Chicago

Courses:

BIOL 300 Cell Physiology

BIOL 323 Human Physiology

BIOL 455 Marine Biology

BIOL 424 Animal Physiology

Research Interests:

Epithelial ion and transport and gas exchange

The research in my lab will encompass an area of comparative physiology that spans from the cell to the organism, with a central theme of organismal function. My major area of interest is the regulation and control of transport across amphibian skin, including: epithelial Na+ transport; the interactions of Na+ and H+ transport in tight epithelia and their roles in intra- and extracellular acid-base regulation; integrating the role of a minority cell type (i.e., the mitochondria rich or intercalated cell) with that of the cells that form the majority in Na+ transporting epithelia; and integrating the physiological processes of gas exchange, ion transport and acid flux that occur in tandem across amphibian skin.

My research examines the regulation of Na+ transport, in terms of physiological function, mRNA expression and translation into protein, in frog skin under different natural conditions. Many classic studies have utilized frog skin as a model epithelium, however, these studies are often carried out under non- physiological/ecological conditions in order to elucidate the general mechanisms of epithelial Na+ transport. However, frog skin functions differently in physiologically relevant conditions and in a manner that suggests a potential link between cutaneous Na+ uptake and acid-base balance. I am interested in cutaneous Na+ transport, not as an isolated event, but as one of many concurrent events occurring across and within the epithelium. Physiological processes inherent in amphibian skin include ion transport, acid-base balance, gas exchange and water balance. Research in my lab will address to what extent these concurrent processes influence or constrain one another under differing environmental conditions or during times of natural stresses. Both physiological and molecular techniques will be used. Many of the putative membrane transporters and cellular proteins involved in these processes have been identified and cloned in other species, making it feasible to probe for the amphibian version of these transporters. These tools can then be used to examine concurrent changes in physiological function, gene expression and translation when the animals are subject to different environmental conditions. This type of work is very compatible for collaborative work involving students at all levels and I welcome all inquiries.

Publications:

  1. Talbot, C. R. 2002. The effects of perfusion of the cutaneous vasculature on sodium uptake in isolated frog skin. Journal of Comparative Physiology B. 172 (3): 209-216.
  2. Stiffler, D.F. and C.R. Talbot. 2000. Exchanges of oxygen, carbon dioxide, nitrogen and water in the ceacelian Dermophis mexicanus. Journal of Comparative Physiology B. 170:505-509.
  3. Rochelle, L.G., D. C. Li, H. Ye, E. Lee, C.R. Talbot and R. C. Boucher. 2000. Distribution of ion transport mRNAs throughout murine nose and lung. Am. J. Physiol. Lung Cell. Mol. Physiol. 279:L14-L24
  4. Talbot, C. R., D.G. Bosworth, E.L. Briley, D. A. Fenstermacher, S. E. Gabriel, R.C. Boucher and P.M. Barker. 1999. Quantitation and localization ENaC subunit expression in fetal, newborn and adult mouse lung. American Journal of Respiratory and Critical Care Medicine. 20(3):398-406
  5. Talbot, C. R. 1999. Species specific variations in ENaC expression in mammalian respiratory epithelia. In "Amiloride-Sensitive Sodium Channels: Physiology and Functional Diversity" Current Topics in Membranes, vol. 47, D. J. Benos, Ed. pp. 197-217.
  6. Hummler, E., P. Barker, C. Talbot, Q. Wang, C. Verdumo, B. Grubb, J. Gatzy, M. Burnier, J.-D. Horisberger, F. Beermann, R. C. Boucher and B. C. Rossier. 1997. A mouse model for the renal salt-wasting syndrome pseudohypoaldosteronism. Proceedings of the National Academy of Sciences USA. 94:11710-11715.
  7. Farman, N., C.R. Talbot, R. Boucher, C. Canessa, B. Rossier, and J.P. Bonvalet. 1997. Non-coordinate expression of a, b and g subunit mRNAs of the epithelial sodium channel along the rat respiratory tract. American Journal of Physiology: Cell. 41:C131-C141.
  8. Burch, L., C. Talbot., M.R. Knowles, C. Canessa, B. Rossier and R.C. Boucher. 1995. Relationship of Na+ channel subunits to pathogenesis of CF airway disease. American Journal of Physiology: Cell. 38:C511-C518.
  9. Feder, M.E., R.J Gonzalez, T. Robbins and C.R. Talbot. 1993. Bulk flow of the medium and cutaneous sodium uptake in frogs: Potential significance of sodium and oxygen boundary layers. Journal of Experimental Biology. 174:235-246.
  10. Talbot, C. R. and M.E. Feder. 1992. Differences among cutaneous surface area, cutaneous mass, and body mass in frogs: A reappraisal. Physiological Zoology. 65(6):1135-1147.
  11. Talbot, C. R. 1992. Regional variation and control of cutaneous gas exchange in the bullfrog. Respiration Physiology. 89:261-272.
  12. Talbot, C. R. and D.F. Stiffler. 1992. Cutaneous ion exchange, and renal and extra-renal partitioning of acid and ammonia excretion in larval Ambystoma tigrinum following ingestion of ammonium salts. Journal of Comparative PhysiologyB 162(5):416-423.
  13. Talbot, C. R. and D.F. Stiffler. 1991. The effects of hypoxia on acid-base balance, blood- gases, catecholamines and cutaneous ion exchange in the larval tiger salamander Ambystoma tigrinum. Journal of Experimental Zoology. 257(3):299-305.
  14. Stiffler, D.F., M.L. DeRuyter and C.R. Talbot. 1990. Osmotic and ionic regulation in the aquatic caecilian Typhlonectes compressicauda and the terrestrial caecilian Ichthyophis kohtaoensis. Physiol. Zool. 63(4):649-668.

Presentations at meetings (since 1997):

*student researcher working in my lab

  1. Paasch*, L. and C. R. Talbot. 2001. Behavioral responses to environmental solute concentration in dehydrated Pacific Treefrogs. (FASEB Journal 15:A413)
  2. Villaneuva*, C. and C. R. Talbot. 2001. Time dependent changes in cutaneous sodium transport in leopard frogs (Rana pipiens). (FASEB Journal 15S:13)
  3. Talbot, C. R. 2000. Regulation of cutaneous sodium transport. Physiological Ecology meetings, White Mountain Research Station, Bishop, CA.
  4. Talbot, C. R. 2000. International Symposium in association with the Ph.D. course in ion channel patch clamp electrophysiology. August Krogh Institute, Copenhagen, Denmark. Title: Regulation of cutaneous sodium transport in the leopard frog Rana pipiens.
  5. Talbot, C. R., J. Goldstein* and S. D. Hillyard. 2000. Acclimation in deionized water increases the NPo of the epithelial sodium channel in frog skin. Experimental Biology 2000. (FASEB Journal 14:A593).
  6. Talbot, C. R. and M. Mangoba*. 2000. Free access to the aquatic medium reduces the degree of regulation of cutaneous Na+ transport in frogs. Experimental Biology 2000. (FASEB Journal 14:A338).
  7. Talbot, C. R. and Hillyard S.D. 1999. Upregulation of amiloride-sensitive sodium current across the skin of the leopard frog, Rana pipiens. Fifth International Congress of Comparative Physiology and Biochemistry. (Comparative Physiology and Biochemistry 124S:108).
  8. Talbot, C. R., R.C. Boucher and P.M. Barker. 1997. Developmental expression of the amiloride-sensitive epithelial sodium channel (ENaC) subunits in fetal mouse lung. Experimental Biol. '97. (FASEB J. 11:A561)
  9. Talbot, C. R., N. Farman, P.M. Barker, R.C. Boucher. 1997. Localization of a-, b-, and g- ENaC in human, rat and mouse respiratory epithelium. American Physiological Society meeting on "The Physiology and Functional Diversity of Amiloride-Sensitive Na+ Channels: A New Gene Superfamily". (The Physiologist. 40:A21).